篇名:
豬博德氏菌、巴氏桿菌、胸膜肺炎放線桿菌、丹毒絲狀菌及假性狂犬病五合一不活化混合疫苗免疫小鼠對豬丹毒絲狀菌攻擊之安全與免疫效力Satety and Potency of Five-in-one Inactivated Vaccine Against Erysipelothrix rhusiopathiae Challenge in Mice
作者:
陳 清;柯浩然;邱資峰;賴俊雄;姜寶仁;林士鈺
中文摘要:
豬博德氏菌、巴氏桿菌、放線桿菌、丹毒絲狀菌及假性狂犬病五合一不活化混合疫苗,接種試驗小鼠,其結果免疫注射後小鼠會損失。在本試驗中試製五合一不活化菌苗及單價豬丹毒不活化菌苗,依目前國家檢定標準及筆者等之改進方式作一比較試驗。五合一不活化菌苗,使用小鼠為材料,重複測試其抗豬丹毒效力之結果,得知如以10倍稀釋菌苗0.5 mL 腹腔免疫注射方法,其防禦指數分別為102.24 ( 263 ) 及102.7 ( 501.2 ),而相同劑量如以皮下注射免疫方法其防禦指數卻很低,或無法測知。以目前國家檢定劑量0.1 mL 皮下注射方法免疫者,其防禦指數分別為100.82 ( 6.6 )及100.9 ( 7.9 ),但同劑量如改以腹腔免疫注射者,其防禦指數分別提升為101.31 ( 20.4 )及104.16 ( 14454.4 ),雖較皮下注射表現佳,惟因濃度高供試小鼠有損失( 21% ) 與不穩定。至於試製單價不活化丹毒菌苗之力價試驗,10倍稀釋菌苗0.5 mL 腹腔注射免疫者其防禦指數可達102.89 ( 977.2 ),相同劑量皮下注射免疫者則僅有101.15 ( 14.1 )。以國家檢定劑量0.1 mL 腹腔免疫者之效力亦較皮下免疫注射者為佳。至於免疫各組小鼠血清之發育凝集抗體價如表所示成績。根據本試驗成績,建議修正豬丹毒不活化菌苗檢定標準時,將效力試驗皮下免疫注射方法及免疫劑量修正為將不活化菌苗10倍稀釋液0.5 mL 腹腔免疫注射14日後攻毒,觀察二週,試驗組及對照組分別加以記錄,依Reed & Muench方法計算其LD50,結果試驗組之防禦指數須≧101 ( 10 )。本改進之檢定方法易於實施且結果將更為穩定與確實。
英文摘要:
A five-in-one inactivated vaccine consisting of Bordetella bronchiseptica (Bb), Pasteurella multocida (Pm), Actinobocillus pleuropneumoniae (App), Erysipelothrix rhusiopathi- ae (Er) and pseudorabies virus was used to immunize mice. In this research, both the five-in-one inactivated vaccine and the monovalent Erysipelothrix rhusiopathiae bacterin were tested with two methods: the National Standard Bioassay Regulations (NSBR) and our modified method. Po- tency tests in duplicate against Erysipelothrix rhusiopathiae with 0.5 mL of the 10 times diluted five-in-one vaccine in mice, administered by intraperitoneal inject (IP) method had protection indexes of 102.42 (263) and 102.7 (501.2), respectively. However, the same dosage injected subcu- taneously had very low or undetectable protection. When using the NSBR method of administer- ing with 0.1 mL by subcutaneous injection (SC) resulted in a protection index of 100.82 (6.6) and 100.9 (7.9). The protection indexes were increased to 101.31 (20.4) and 104.16 (14454.4) respective- ly. When the same dosage was administered by IP method. Obviously, these results indicated that vaccination using the IP method was more effective than the SC method. There were some losses of mice (21%) and unstable of safety, that might be caused by the higher concentration of the bacterin. On the other hand, the potency test in mice for the trial of Eysipelothrix rhu- siopathiae monovalent bacterin with 10-1 0.5mL administered by IP method had a protection index of 102.99 (977.2).The same dosage administered by SC method had a protection index of only 101.15 (1.41). When using the NSBR procedure with a dosage of 0.2 mL (1:1 diluted), the potency test resulted from the IP method was also better than that from the Sc method. Based on these find- ings, we suggest that the potency test procedure for the Erysipelothrix rhusiopathiae bacterin needs to be modified according to the injection method and the dosage used. The suggested pro- cedures are as follows. First, the inactivated bacterin should be diluted 10 times. Second, ad- minister 0.5 mL of the diluted bacterin by using the IP method. After 14 days, challenge both the experimental and control groups of mice and observe for two weeks. During this period, record the results of both the immunized and the control groups. Then, calculate the LD50 using the Reed & Muench method. The result of protection index for the immunize group should be equal to or more than 101.0 (10). This modified method is not only easier to follow but more accurate to ob- tain the results
備註:
年份:西元 2002 年
期別:第 38 期
詳細內容:
Swine erysipelas (SE) is caused by Eyrsipelothrix rhusiopathiae (ER.), which is dis- tributed worldwide in pig farms. This organism causes acute septicemia, urticarial lesions, endo- carditis and polyarthritis in pigs [11]. It also causes polyarthritis in sheep, lambs and serious death loss- es in turkey. This organism has been isolated from the body organs of many species of wild and domestic mammals and birds [13,14,15]. In humans, Er.. causes erysipeloid, a local skin lesion that oc- curs mainly as an occupational disease in people [6]. The organism can occasionally be isolated from human cases of endocarditis and rarely cause acute septicemia disease [16].  Since the attenuated vaccine and antibiotic were developed, the outbreak of swine erysipelas has been remarkably reduced [12]. Inevitably, there are always some cases reported in Taiwan [2]. On the other hand, according to the report by Lu et al . [7], antimicrobial additives containing amoxicillin and chloramphenicol used in feed could be interferred with the immunity effects of the at- tenuated vaccine in pigs. Therefore, in order to make the monovalent or polyvalent inactivated bac- terins will be more acceptable to the farmers, the safety and immune efficacy evaluation and explo- ration of the bacterins are essential.
附件下載:
附件名稱:
五合一不活化混合疫苗免疫小鼠對豬丹毒絲狀菌攻擊之安全與免疫效力
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