篇名:
水禽雷氏桿菌症不活化多價菌苗之研發與應用Development and Application of a Trivalent Inactivated Bacterin against Riemerella anatipestifer Infection
作者:
喻昭芳*;黃金城;趙磐華
中文摘要:
本研究的目的為開發具保護力的水禽雷氏桿菌症3價不活化菌苗,以符合市場防疫需求。首先進行菌株的蒐集、選定與鑑定,完成蒐集共21種血清型菌株並製備高免血清,以利後續快速檢測鑑定田間流行型別,並初步選定近年流行第1、2及6血清型為未來研究菌苗株。接著著手菌苗最適生產條件建立,及各種免疫效力提昇試驗,由結果可知不同培養基與培養時間對最終菌苗效力影響不大,2倍與10倍稀釋劑量對鴨隻保護效果不佳,多次菌苗效力試驗防禦指數成績皆可達多種不活化菌苗國家檢定標準。最後使用ELISA檢測鴨血清抗體方法建立,由預測趨勢線顯示,免疫3價菌苗後可引起鴨隻體內3型高抗體力價。
英文摘要:
The purpose of this study is to develop a trivalent inactivated bacterin against Riemerella anatipestifer (RA) infection. At first, we have finished the collection, selection and identification of 21 serotypes of RA strains. The hyperimmunized sera against 21 serotypes of strains were also produced by using the rabbit models and they will be used for fast identification of field strains in the future. The strains including serotypes 1, 2 and 6 were preliminarily selected to develop a trivalent bacterin. We have also established various culture methods, and performed the comparison of different results to determine the best conditions for the induction of immune efficacy. The results showed that there is no significantly difference on potency tests of the trivalent bacterin prepared from different culture time or media. The 1/2 and 1/10 dosages could not provide enough protection against challenge. Several potency tests have shown that the most protection indexes of the bacterin have reached the national standards of animal drug inspection. The development of an ELISA kit for detecting duck serum antibodies against RA antigens has shown sufficient sensitivity and susceptibility.
備註:
年份:西元 2008 年
期別:第 43 期
附件下載:
附件名稱:
006
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