豬第二型環狀病毒感染性質體之建立
Establishment of Infectious Clone of Porcine Circovirus Type 2

作者:     王 ?;邱芮瑜;黃天祥;李 璠;鍾明華;張惟茗
中文摘要:     豬第二型環狀病毒(porcine circovirus type 2, PCV2),為引起豬離乳後多系統消耗性綜合症(postweaning multisystemic wasting syndrome, PMWS)PMWS之主要病原。PCV2常與其它病原混合感染,增加單獨分離此病毒的困難度,再者分離的PCV2病毒在細胞培養時其力價不高。本次論文主要目的是構築具感染性PCV2質體,利用細胞培養建立PCV2量產模式,作為穩定單純的病毒來源。將感染性PCV2質體轉染細胞後產生之病毒加以收集,並測試其力價,最高可為105.5 TCID50/mL。另一方面,轉染質體DNA之劑量的不同並不造成新生病毒力價的顯著差異。
英文摘要:     Porcine circovirus type 2 (PCV2) is associated with post-weaning multisystemic wasting syndrome (PMWS). PCV2 is an important infectious disease in swine and occasionally co-infects hosts with other pathogens. PCV2 is difficult to isolate from field samples, and is maintained at low titers in vitro. In this study, we have constructed the infectious clone of PCV2 to generate stable virus. The infectious clones were transfected into PK-15 cell line to generate new viral particles. The highest titer of newly generated viral particles was 105.5 TCID50/mL after a sixfold passage. In addition, there was no statistically significant differences between virus titer and infectious clones of various transfect dosages.
備註:     年份:西元 2009 年
期別:第 44 期
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附件名稱 06王-~1    
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