篇名:
豬假性狂犬病gE基因缺損不活化疫苗之免疫效力Efficacy Evaluation of the gE-Deleted Pseudorabies Inactivated Vaccines
作者:
姜寶仁;鍾明華;邱資峰;柯浩然;陳 清
中文摘要:
以本土性豬假性狂犬病gE及TK雙基因缺損R23病毒株及gE自然缺損Bartha病毒株研製兩種不活化疫苗,對家兔之免疫保護效力均可通過國家檢定標準。兩種不活化疫苗分別在4週齡無特定病原小豬免疫兩次,結果顯示豬隻施打R23及Bartha疫苗後之平均中和抗體力價分別為1:53.3及1:256倍,再以野外強毒株攻擊後,免疫組中和抗體急劇升高,排毒天數及發熱天數均比對照組少。同時免疫豬隻的血清以ELISA kit檢驗結果均顯示gE抗體陰性,攻毒後轉為陽性。結果顯示兩者gE不活化疫苗對豬隻具有保護力,同時亦具有區分gE抗體的功能。
英文摘要:
SUMMARY The gE and TK double deleted R23 strain of pseudorabies virus (PrV) constructed in Taiwan and a natural gE-deleted Bartha strain were used to develop inactivated vaccines. The protective efficacy of the two vaccines in rabbits could fulfill the requirement of national drug assay. The SPF pigs aged at four weeks old were vaccinated either with the R23 or Bartha vaccine twice at an interval of 3 weeks. Results revealed that the mean neutralizing antibody titers of R23- and Bartha- immunized pigs were 1:53.5 and 1:256, respectively. The neutralizing antibody titers of the vaccinated pigs rose sharply following challenge with the wild type virus. The mean duration of fever and virus excretions in the vaccinated pigs were decreased compared to the control pigs. The specific antibodies against gE in all pigs were maintained negative after two time vaccinations, and seroconverted to positive post challenge with the wild type virus. In summary, these two vaccines provided good protective efficacy in pigs and could be used for differentiating vaccinated from infected pigs.
備註:
年份:西元 2001 年
期別:第 37 期
詳細內容:
豬假性狂犬病(Pseudorabies; PR)主要引起母豬流死產與幼齡仔豬死亡,為台灣重要豬隻傳染病之一。由於豬隻在恢復後,容易形成潛伏性感染,使該病不易撲滅,因而對養豬業造成嚴重的經濟損失。為了減少本病所引起的經濟損失,應將PR驅除在豬場之外。目前國內使用的PR不活化疫苗,其所刺激產生之抗體與野外病毒感染所產生抗體無法區別,因此也就無法淘汰野外病毒感染之豬隻,進行PR清除的工作。 自從gE-ELISA Kit問世後,歐美國家陸續利用gE基因缺損疫苗配合gE-ELISA Kit 進行PR撲滅工作〔15, 16, 18, 19〕,成效良好。隨著WTO的入關,如何提昇畜牧業的競爭優勢,除了減少疾病的發生降低成本外,特定疾病的清除亦不失為檢疫防堵的條件,相信可減少對台灣養豬業的衝擊。 為了探討本土性假性狂犬病gE及TK雙基因缺損R23株病毒〔1, 2〕與gE基因自然缺損Bartha株病毒不活化疫苗之免疫效力〔10, 12, 13〕,研製兩者不活化疫苗進行各項試驗,以期早日供應國內需求。
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附件名稱:
豬假性狂犬病
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