篇名:
抗生物質殘留種類之篩選與辨別的檢測系統評估Assessment on Screening and Differential Detection System for Categorized Antimicrobial Substance Residues
作者:
林文華;翁麗珍;張春梵;張正明;宋華聰
中文摘要:
抗生物質殘留分析常見採行瓊膠擴散試驗,並藉由微生物生長抑制進行篩選;本研究報告檢測試驗系統任務涵蓋初步篩選與後續辨別等二部分。初步篩選檢測研究採用菌膠呈色法之市售許可合格商品PremiRTest 檢測套組,適用動物產品檢體之常用抗生物質殘留檢測,合計九類34種抗生物質涵蓋範圍的檢測結果顯示:乙內醯胺、四環素、胺基配醣?、巨環素和磺胺劑等類的靈敏度檢測效能足以檢出法定最高殘留容許量濃度。後續辨別檢測研究採用四種培養基菌盤測試法之市售許可合格商品,默克公司抗生物質測試瓊膠培養基的編號暨酸鹼度[I] 10663 pH 6.0,[II] 105270 pH 7.2,[III] 10664 pH 8.0,與[IV] 10664 pH8.0等,並於[I,II,III]與[IV]培養基分別接種枯草桿菌Bacillus subtilis ATCC 6633芽孢與微小球菌Sarcina lutea ATCC 9341等敏感菌株;進行各類抗生物質族群類別之篩選和辨別的檢測結果顯示為四種菌盤敏感性高低順序:乙內醯胺類 IV >I>III >II (或 II> III);四環素類 I> II> III> IV;胺基配醣?類 III >II >I >IV;與巨環素類 IV> III> II> I。磺胺劑類檢測另加三甲?氨嘧啶提高敏感度。   綜合本研究報告結果顯示,抗生物質殘留檢測之篩選與辨別的整合型系統建置應可有效達成實際任務目標。初步篩選型菌膠呈色法適合建置食品供應商的自發性預行檢測,後續辨別型四種培養基菌盤測試法適合建置主管機關的整合型篩選辨別檢測系統;檢測效能靈敏度最佳限值符合衛生署公告之動物用藥殘留標準的殘留容許量,有效確保動物產品符合最高殘留容許量法定標準。
英文摘要:
The detection of antibiotics residue is generally analyzed by the growth inhibition ring of sensitive microbial strains revealed on agar plate diffusion test. An integrated efficient detection system of initial screening detection and subsequent differential detection is performed in this research report. The initial screening detection adopts microbial gel coloring method (MGCM) with permitted commercial package on antibiotic residue detection over various animal products. The PremiRTest kit by Dutch DSM food company effectively covers 9 antibiotics classes with 34 drugs of routine usage including beta-lactams, tetracyclines, aminoglycosides, and sulfa drugs. Our detection result with PremiRTest kit revealed superior detection sensitivity on antibiotic residue classes as to be more sensitive than allowed maximal residue quantity of government regulation. Moreover, the subsequent differential detection adopts four plate test (FPT) with permitted microbial media and sensitive microbial strains on antibiotic residue detection over various animal products. Merck antibiotic test agars (MATA) are respectively applied on four plates with catalog numbers of [I] 10663 pH 6.0, [II] 105270 pH 7.2, [III] 10664 pH 8.0, and [IV] 10664 pH 8.0 in which plates [I, II, III] are inoculated with Bacillus subtilis ATCC 6633 and plate [IV] with Sarcina lutea ATCC 9341. Screening and differential detection among several routine antibiotics classes can be accomplished by respective sensitivity ranking pattern of antibiotic residue with FPT inhibitory ring sizes including beta-lactams [IV >I >III >II (or II> III)], tetracyclines [I >II >III >IV], aminoglycosides [III >II >I >IV], macrolides [IV >III >II >I].   In conclusion, the integrated detection system of initial screening detection and subsequent differential detection may be evidently efficient in fulfilling practical goal of antibiotic residue detection over various animal products. Initial screening detection of MACM may be established as voluntary pre-detection by food product suppliers. Subsequent differential detection of FPT may be established as surveillance detection as of integrated detection system by regulatory institutions. Methodology performance of best detection limit is effectively within allowed veterinary drug residue limit supervised by the Department of Health which in turn efficiently assures regulatory compliance on legal standards in allowed maximal antibiotics residue quantity.
備註:
年份:西元 2006 年
期別: 第 41 期
詳細內容:
最近幾年國內消費者經過口蹄疫(FMD)、牛海綿狀腦部病變(BSE)、重症急性呼吸症候群(SARS)、戴奧辛和禽流感等危機的不斷考驗後,對於食品安全衛生的信心不足;因此,針對於現代牧場經營密集使用數量龐大的藥物,呈現不肯定的態度,針對食品安全的改革提昇趨向非常嚴格的要求態度。  動物專用藥品的眾多種類品項暨巨大應用數量,顯然成為食品安全的高度相關影響因子;依據理論任何投藥動物路徑均可導致殘留,合法投藥抗生物質於養殖場動物防止疾病的爆發與蔓延,常因缺乏明顯有效停藥時間、未依指定藥劑治療、飼料污染或非法使用未許可藥物,與使用未核准藥物(9,17,19)等,導致殘留出現於動物來源產品如牛乳、肉和蛋等,或是排出於體外危害食物鏈供需公衛安全與惡化耐藥性環境安全問題(3,24)。  食品的藥物殘留檢測策略的一般程序【篩選,辨別,定量,確認】;然而,現行檢測違反最高殘留容許量,常須依據事前指定已知的殘留藥物種類採用單種色層分析法定量確認;因此,導致忽略篩選辨別的有效程序,無法滿足繁多藥物種類與互異操作條件的分析需求與資源節約,容易遺漏其他未經事前指定檢測的藥物種類,再者,具備HPLC和LC-MS/MS 高科技儀器實驗室屈指可數;故而依據指定種類的檢測策略常會誤導使用者造成藥物濫用猖獗,因為使用者事前知悉檢測項目策略即可規避藥物監控與違法改用其他等效的藥物種類,致使實驗室測試確認的結果報告嚴重瑕疵與品質保證受到懷疑。  篩選的分析法通常提供經濟型定性與不定性檢測結果。微生物學方法通常較適於大量檢體篩選,具備經濟方便與藥物種類範圍寬廣的特性(1,23),可以排除大量偽陽性檢體,檢出特異族群藥物種類,續行前述陽性檢體辨別定量確認,連結整合建置完成整體分析檢測系統,解決現行抗生物質殘留檢測的欠缺。因此本研究報告主要利用微生物學篩選和辨別抗生物質的檢測評估,作為畜禽水產品抗生物質殘留的日常例行性篩選和辨別,以確保品質符合法令規定的最高殘留容許量標準要求規格。
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抗生物質殘留種類之篩選
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