作者:     喻昭芳;黃金城
中文摘要:     為瞭解自行研發的水禽雷氏桿菌症第1、2和6血清型3價不活化菌苗大量產製後應用於田間水禽場,其菌苗對鴨及鵝隻的保護力是否與之前實驗室內小批進行的結果一致。將量產1萬劑量菌苗分別實施於北京鴨場,番鴨場與白羅曼鵝場,適齡時帶回本所攻毒,以Beherens-Karber法計算LD50防禦指數評估菌苗效力。試驗成績均可達到其他不活化菌苗國家動物用藥品檢定標準,顯示免疫本菌苗後經人工感染可產生良好的保護效果。有鑑於乾淨試驗動物難尋,另嘗試使用小白鼠測試,結果顯示小白鼠免疫3價菌苗後,血清凝集抗體及ELISA抗體價均可快速爬昇,免疫後攻毒成績亦說明人工感染可成立。
英文摘要:     To investigate the potency of a trivalent bacterin containing serotypes 1,2 and 6 against Riemerella anatipestifer infection, a bacterin was manufactured on a large scale and tested in the laboratory and on waterfowl farms. The Beherens-Karber method was used to evaluate the protection index based on LD50 from vaccinated and control animals. The results showed that all of the protection indices achieved the national standards of animal drug inspection. This bacterin was found to provide protection against experimental homologous serotype challenges. We performed efficacy tests as well as detection of sera antibody in mice, the results indicating that vaccinated mice exhibit a strong response to immunogens against Riemerella anatipestifer.
備註:     年份:西元 2009 年
期別:第 44 期
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08 喻昭芳-水禽雷氏桿菌症3價不活化菌苗之效力試驗    
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