篇名:
應用聚合?連鎖反應-限制酵素切割圖譜(PCR-RFLPs)檢測市面販售犬、貓飼料中肉品種類來源Examination of Meat Components in Commercial Dog and Cat Feed by Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms (PCR-RELPs) Technique
作者:
王咸祺;李淑慧;張天傑;王孟亮
中文摘要:
本實驗目的為利用聚合?連鎖反應-限制酵素圖譜 (PCR-RFLPs) 檢測犬貓飼料中肉品種類來源。由於牛海綿狀腦病 (俗稱狂牛症) 之類的神經性疾病可能經由被污染的食物所引起,因此檢測動物飼料中肉品來源,對於獸醫公共衛生而言極為重要的。本實驗的結果利用聚合?連鎖反應技術將動物細胞內線體的部份細胞素 (cytochrome) b基因之片段增幅,之後再以限制酵素Alu 1及Mbo 1切割這些PCR產物,進而達到區分不同種類動物肉品來源之目的。本實驗共檢測八種市面上所販售的犬飼料以及貓飼料,結果顯示所有市售貓飼料部分所檢查之結果,有八分之五的品牌中有雞肉來源的成分。
英文摘要:
It has been shown that certain slow neurological diseases such as bovine spongiform encephalopathy (also known as “mad cow” disease) could be transmitted through contaminated food intake by animals; therefore, the examination of meat components in commercial feeds is important for the control of the disease in public health. The combination of polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) technique was applied to examine the meat components in dog and cat commercial feeds. The partial nucleotide sequence (359 bp) of animal mitochondrial cytochrome b (cytb, CYT) gene was amplified by PCR and then digested with restriction enzyme Alu 1 or Mbo 1. In this work, eight brands of commercial dog and cat feed available in Taiwan were examined. All brands of dog feeds that were tested contained meat from four different animals (cattle, pig, goat and ckicken). In cat feeds, the chicken meat was found in five out of eight brands.
備註:
年份:西元 2003 年
期別:第 39 期
詳細內容:
Transmissible diseases caused by abnormal prions can affect different species of animals by eating contaminated food [6]. The use of simple and sensitive techniques to examine food components can avoid the spread of some slow infectious diseases, such as scrapie, bovine spongiform encephalopathy (FSE) [6]. Several mitochondrial DNA genes, such as conserved sequence blocks (CSBs), 16S ribosomal RNA gene and cytochrome b (cytb) gene, have been used to study animal evolution [3-5]. The cytb gene is among the most extensively sequenced genes to date among vertebrates. By PCR, the cytb gene was amplified and sequenced to compare the intra-species and inter-species differences of animals including amphibians, birds, fishes, and mammals [4, 5].  In this study, we used the PCR-RFLP technique to differentiate meat components among cattle, goat, pig, and chicken. The 359 bp fragments of cytb gene were generated and distinct digestion patterns of these DNA fragment were observed after Alu 1 or Mbo 1 treatment. We investigated the components of commercial dog and cat feed in Taiwan. The results demonstrated that all tested dog feed was mixed food and the component in cat feed was relatively consistent with that on the label.
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附件名稱:
應用聚合?連鎖反應-限制酵素切割譜(PCR-RFLPs)檢測市面販售犬、貓飼料中肉品種類來源
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