篇名:
海鱺發光菌細胞外產物抗原性之研究The antigenicity of extracellular products of Photobacterium damselae subsp. piscicida in cobia (Rachycentron canadum)
作者:
涂堅;邱芮瑜;張正芳;黃淑敏;張惟茗;趙磐華
中文摘要:
本研究主要目的為篩選海鱺發光菌其細胞外產物中具有抗原性的蛋白部份供疫苗發展用。此抗原性蛋白乃透過細菌的細胞外產物進行西方轉印法以海鱺抗發光菌的特異抗血清加以確認,再將此抗原性蛋白進行質譜分析。將已知氨基酸序列胜?片段進一步與基因庫中現存序列比對,發現一個分子量約43 kDa蛋白,具有脂肪?功能,其生物功能尚待進一步探討。
英文摘要:
The purpose of this study is to screen the major antigenic protein moieties of the extracellular products of Photobacterium damselae subsp. piscicida in cobia for vaccine development. The antigenic proteins are verified through Western blot of the extracellular products reacted with the cobia antiserum against the bacteria. The collected antigenic bands were sent for mass spectrometry anaylysis and the peptide masses were compared with the protein sequences in GenBank. One 43 kDa protein was identified as a bacterial lipase and further research on its biological function will be done.
備註:
年份:西元 2008 年
期別:第 43 期
附件下載:
附件名稱:
003
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