篇名:
雞飼料中安保寧與衣索巴同時檢測方法之建立Simultaneous Determination of Amprolium and Ethopabate in Chicken Feeds
作者:
劉雅方;黎南榮;蘇杰夫;林士鈺
中文摘要:
本研究開發以高效液相層析法同時檢驗雞飼料中安保寧(amprolium)與衣索巴(ethopabate)之方法。檢驗以甲醇萃取、過濾,並經固相萃取管柱淨化,最後利用高效液相層析儀分析。所用之移動相為甲醇-水(40:60)含辛烷礦酸及乙胺,層析管柱為Cosmosil 5 C8-MS,於270nm 檢測。飼料樣品添加安保寧最終濃度2.5ppm 及1.25ppm 時,回收率分別為92.3%及95.3%,變異係數 1.4% 及 0.7%。添加衣索巴最終濃度 0.4ppm 及 0.2ppm 時,回收率分別為 90.0%及 83.9%,變異係數為 7.1% 及 4.2%。安保寧與衣索巴利用本方法之最低檢出界限分別是 0.025ppm 與 0.015ppm 。
英文摘要:
A reversed-phase high performance liquid chromatographic ( HPLC ) method was developed for simultaneously analysing amprolium and ethopabate in chicken feeds. The drugs were extracted from samples with methanol and were filtered. The methanolic filtrate was cleaned up by solidphase extraction. HPLC was done on the Cosmosil 5 C8-MS column with methanol-water ( 40 : 60) ,containing octanesulfonic acid, triethylamine and glacial acetic acid, as mobile phase. The analytes were detected at 270 nm. Average recoveries for amprolium-fortified samples at 2.5 and 1.25 ppm were 92.3% ( coefficient of variation, CV, 1.4% ) and 95.3% (CV, 7.1% ), respectively. Average recoveries for the ethopabate-fortified samples at 0.4 and 0.2 ppm were 90.0 % ( CV, 7.1 % ) and 83.9 % ( CV, 4.2% ) respectively. The levels of detection limit for amprolium and ethopabate were 0.025 ppm and 0.015ppm, respectively
備註:
年份:西元 1999 年
期別:第 35 期
詳細內容:
安保寧(amprolium)與衣索巴(ethopabate)為合成抗原蟲劑,依據行政院農業委員會之「飼料添加物使用準則」〔1〕規定,安保寧與衣索巴以125~250ppm 與8~16ppm 之比例混合添加於雞飼料中,可作預防球蟲病使用。飼料中安保寧之檢測方法有呈色法〔3〕、正相高效液相層析法〔5〕、螢光法〔15〕等。飼料中衣索巴之分析方法包括呈色法〔4〕、高效液相層析法〔14〕、氣相層析法〔3〕等。安保寧與衣索巴雖混合添加於飼料,但卻不見於飼料中同時檢測方法之文獻,且上述之個別分析方法中,呈色法與螢光法需經衍生化反應,色層分析法需經多種溶劑萃取或使用傳統的管柱層析分離,過程複雜費時。本實驗目的為進行雞組織中該二種藥物殘留與藥物動力學研究之準備試驗,研發同時檢測雞飼料中安保寧與衣索巴之定性定量方法,以快速準確分析於飼料中之含量。
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附件名稱:
雞飼料中安保寧
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