篇名:
飼料內卡巴得、歐來金德、富來頓、乃挫夫喃頓及乃託文之高效液相色層分析法High-Performance Liquid Chromatographic Determination of Carbadox, Olaquindox, Furazolidone, Nitrofurazone, and Nitrovin in Feed
作者:
林士鈺;鄭秀蓮
中文摘要:
為同時分析飼料內卡巴得、歐來金德、富來頓、乃挫夫喃頓及乃託文,利用C8矽膠層析管在梯度分析條件下,可得完全分離之層析圖。梯度分析主要以每min5% acetonitrile之線性梯度。五種飼料添加物檢量線之相關係數(Y)均在0.999以上且相對標準偏差0.3%以內,僅5ppm乃託文大些(6.7%)。飼料先加水處理後,以95% dimethylformamide 於室溫中過夜萃取,再通過三氧化二鋁管柱。低濃度飼料回收率分別為102.0, 94.6, 97.4, 110.6及66.0%,高濃度則為114.0, 99.1, 109.9及62.7%。
英文摘要:
A high-performance liquid chromatography with gradient programming method was developed to determine the amount of carbadox (CBX), olaquindox (OLQ), furazolidone (FZ), nitrofurazone (NF), and nitrovin (NTV) in feed simultaneously. Complete separation of the drugs was obtained using a C8 silica gel column with gradients of acetonitrile as mobile phase. The mobile phase used an acetonitrile gradient with an initial hold time of 1 min at 0% acetonitrile, followed by an increase to 50% acetonitrile over 10 min. The correlation coefficients (r) for calibration curves of the five feed additives were greater than 0.999. The relative standard deviations (RSDs) of peak areas from four injections for these drugs at three concentrations were less than 3.0%, although the RSD for NTV at 5 ppm was somewhat large (6.7%). The medicated feeds were extracted by pretreating with water, extracted with 95% dimethylformamide overnight at room temperature, and cleaned up on a column of alumina oxide. Recoveries of CBX, OLQ, FZ, NF, and NTV from low level spiked feed were 102.0, 94.6, 97.4, 110.6, and 66.0% respectively, and from high level spiked feed, they were 114.09, 99.1, 97.3, 109.9, and 62.7%, respectively.
備註:
年份:西元 2003 年
期別:第 39 期
詳細內容:
A project for promoting feed quality sponsored by the Council of Agriculture was carried out at this institute. The project dealt with the assay of drugs in feed, including carbadox (CBX), olaquindox (OLQ), furazolidone (FZ), nitrofurazone (NF), sulfamethazine (SMT), sulfathiazole, and pyrimethamine. Among these feed additives, CBX and OLQ are derivatives of quinoxaline-1.4dioxide, whereas FZ and NF belong to the furan group. NTV is one of the furans allowed for use as a feed additive (6). Although there are official (Chinese National Standard) methods for several of these drugs (Table 1), the methods for CBX and NF are spectrophotometric,which is time-consuming.  Most of the previously published methods for the determinations of OLQ, CBX, NF, FZ, and NTV in feed are concerned only with one (single) drug in feed (1-4, 8, 9, 11, 14, 16-19), and a few experiments were conducted to simultaneously determine two (5, 10, 12, 13, 20, 22), three (15), or four drugs (21). As for this work, there were no reports on the simultaneous determination of residual drugs of OLQ, CBX, NF, FZ, and NTV in feed by high-performance liquid chromatography (HPLC). To simplify the assay procedure, as well as to reduce the workload and increase accuracy, an HPLC with gradient program is presented here for quantitative and simultaneous determination of the above five drugs in feed.
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飼料內卡巴得、歐來金德、富來頓、乃挫夫喃頓及乃託文之高效液相色層分析法
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