篇名:
淡水養殖魚類產氣單胞菌不活化菌苗之研發與應用之評估Development of Aeromonas hydrophila bacterin for freshwater culture fishes and applied evaluation
作者:
柯浩然等
中文摘要:
以吳郭魚分離之產氣單胞菌 (Aeromonas hydrophila) 為種菌株,於腦心浸膏培養基 (Brain heart infusion broth) 30℃培養18 小時後,經集菌及不活化處理與添加佐劑,調製一批不活化菌苗供試。試製之不活化菌苗混於餌料後,以口服方式免疫 (每日2次,連續14 天),試驗魚於免疫14 天後以相同菌株菌液感染攻擊,觀察 14天以相對 活存百分率 (Relative percent survival;R.P.S.) 評估其免疫效力。試驗結果試驗魚池與 田間試驗池之R.P.S 分別為77%及40%,由以上成績顯示,研製之不活化菌苗對 Aeromonas hydrophila感染症具有預防之效益。
英文摘要:
SUMMARY The Aeromonas hydrophila strain isolated from diseased tilapia was used as seed to produce inactivated bacterin. The inactivated bacterin was mixed with meals to immunize the tilapia. The vaccination program was performed two times per day during a period of 2 weeks. The results showed that the relative percent survival (R.P.S.) were 77% in the experimental pools and 40% in the field pools, respectively. This result suggested that the inactivated bacterin had potency to prevent the Aeromonas hydrophila disease.
備註:
年份:西元 1999 年
期別:第 35 期
詳細內容:
細菌性病原菌為魚類疾病中最重要的病原之一,特別是養殖魚類。由於放養密度高,易衍生細菌性疾病造成急速且重大的損失。這些引起魚類細菌疾病之病原菌,曾有學者報告大都是存在水中的好氣性細菌 (Aerobic bacteria),且多屬二次性感染。近年來本省養殖事業日益發達,養殖技術日有改進,因養殖密度日漸提高,因此疾病的發生亦隨之增加。為預防疾病的發生及解決藥物殘留等公共衛生問題,故著手調查本省淡水養殖魚類細菌性疾病,以瞭解疾病的發生情況及病原菌,進而嘗試建立本省相關之細菌種原庫,研發不活化菌苗之應用,探討魚類細菌性菌苗免疫之途徑,落實疾病預防策略,期能減少養殖業者經濟上之損失。
附件下載:
附件名稱:
淡水養殖魚類產氣單胞菌
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