2008年鴨病毒性肝炎病毒分離株之特性與序列分析
Characterization and Sequence Analyses of Duck Hepatitis Viruses Isolated in 2008

作者:     曾俊憲;施雨華;陳韋蘋;簡麗琴;黃天祥;黃金城
中文摘要:     台灣自2008年6月陸續爆發雛鴨大量死亡之疫情,自本次疫情中,共計分離21株病毒,經以序列分析及血清學鑑定結果為:18株第一型鴨肝炎A病毒(Duck Hepatitis A Virus type 1, DHAV-1)及3株ICPI分別為1.75、1.31及0.175之新城病病毒(Newcastle Disease Virus, NDV)。2008年與2003年DHAV-1分離株於VP1之核酸及氨基酸相似性達99.2%及99.6%,與5886疫苗株相似性亦高達92.3%及 95.4%。所有DHV分離株均可被DHAV-1標準血清完全中和,中和指數為5.33至7.67,證實所有病毒株均為DHAV-1。此外以DHAV-1 活毒減毒疫苗肌肉注射免疫1日齡雛鴨,經7日後以2008年之病毒株進行攻毒,結果免疫組100%耐過,對照組死亡率分別為42.9%與69.2%。根據以上分析,本次疫情為本土型DHAV-1再度爆發,並非病毒變異或其他血清型DHV入侵所造成。
英文摘要:     Severe outbreaks of duckling and gosling hepatitis, caused by duck hepatitis virus occurring in Taiwan since June 2008 were studied; 21 strains were isolated from specimens. Among them, 18 samples were identified to be duck hepatitis A virus type I (DHAV-1) and 3 samples were identified to contain the Newcastle disease virus (NDV) after sequence analyses and serologic tests. Based on the virus sequence data, the virus causing the outbreaks was confirmed to be DHAV-1. Similarities of VP1 nucleic acids and amino acids of the 18 isolates were 99.2% and 99.6% compared with the DHAV-1 from 2003 and were 92.3% and 95.4% compared with the 5886 vaccine strain. All these DHV isolates could be neutralized by a standard serum against DHAV-1, and the neutralization indices were between 5.33 and 7.67, indicating that all these isolates belonged to DHAV-1 group. In addition, the one-day old ducklings vaccinated with DHAV-1 vaccine by intramuscular route all survived DHV/2008 challenge on the 7th day post vaccination. The protection rates of vaccinated groups of ducklings were 100%, but the mortalities for non-vaccinated control groups of ducklings were 42.9% and 69.2%, respectively. According to the results mentioned above, we suggest that the outbreaks are caused by the DHAV-1, but not by any mutants or other serotypes of DHV.
備註:     年份:西元 2009 年
期別:第 44 期
附件下載:     
附件名稱
04 曾俊憲-2008年鴨病毒性肝炎病毒分離株之特性與序列分析 8.26.09
行政院農業委員會家畜衛生試驗所版權所有
服務信箱:info@mail.nvri.gov.tw
Tel:0800-068112、02-26212111
FAX:02-2622-5345

免責聲明
 新北市淡水區中正路376號