篇名:
建置實驗動物人道管理自動辨識監測數據系統Deployment of Automatic Identification and Surveillance Data System on Humane Management of Laboratory Animals
作者:
林文華*;張春梵*;張文杰;郭曉芸;林勇成;陳朝欽;高成炎;趙磐華
中文摘要:
建置實驗小鼠籠組自動收集射頻識別與感測數據整合系統係由台大資工無線感測網絡團隊設計開發,硬體系統包括射頻識別感測影音與飲量體溫體重跑輪,應用軟體包括感測數據統計分析採用MatLab程式語言開發,控管介面與資料庫採用Delphi/Java程式語言開發,數據自動登錄與統計分析探採至少同步處理八隻小鼠的有效閾值範圍數據登錄。運用本項準無緊迫型式實驗動物人道管理整合系統,小鼠研究全程31日自動登錄密集數據顯示整體於不同情境的單日表現可見高度變異,個體於不同情境相同社群的單日表現亦見高度變異,推論傳統小鼠實驗誤差因子可能存在日間短期實施對於實驗小鼠的逢機日期晝夜顛倒高度緊迫,本項準無緊迫型式實驗動物人道管理整合系統適為初版解決方案,兼顧同時減輕實驗動物與研究人員的雙方緊迫程度,助益達成實驗動物人道管理基礎目標之最佳數據與最善管理,高度提升鼠隻實驗檢測安全性與數據品質有效性。
英文摘要:
The lab-mice cage-system integration of RadioFrequency IDentification (RFID) and sensor data developed with the Wireless Sensor Network Team in Bioinformatics Laboratory of National Taiwan University comprises both the hardware sensor devices of RFID, audio-video recorder, drink load, body temperature/weight, and jogging wheel and as well the software application systems for conducting statistical data analysis. The databases for the hardware system were developed with MatLab and Delphi/Java programming languages. The software performance is capable of concurrent processing of data generated from 8 or more lab-mice within effective thresholds for data entry. With this nearly no stress lab-mice cage system we have developed a humane regimen for the practice of long-term animal model studies. The data collected using the cage system RFID during a 31-day experiment indicates that each mouse may show a highly variable daily performance according to the experimental parameters used in this study. The measurements of the various physiological responses by this lab-mice cage system of humane management infers that most conventional lab-mice studies introduce inadvertent errors created by opportunistically scheduled dates and day-night inverse stress on lab-mice. This lab-mice cage system of humane management represents a convenient solution for reducing the risks of observer-induced errors caused by researcher-animal interactions. In conclusion, researchers employing our cage system RFID may accomplish the goals of securing optimal data and achieving low stress animal management; this in turn greatly enhances animal trial safety and accuracy of any experiments.
備註:
年份:西元 2008 年
期別:第 43 期
附件下載:
附件名稱:
008
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